Not really, because it’s the indeterminate group watching you. If you say “you” or “you all”, it’s referring to the people interacting with you, not the audience. You have to break the fourth wall to initiate that interaction and make it second person

But streamers sometimes will sometimes, mid conversation with someone else, say “chat, can you get me the link to that?” And continue talking to the other person while waiting for it. They’ll also say “chat is saying I should ask you about XYZ”.

It’s a specific relationship that straddles the line between a second person and third person. They’re also usually not included in first person plural